Fig. 3

OFE attenuated the loss of E-cadherin and abrogated the increase in mesenchymal markers caused by TGF-β1. a SKOV3 cells treated as indicated were assayed by western blot analysis (WB) to assess the expression of epithelial marker E-cadherin. β-actin was used as a loading control. b The quantitative ratios are shown as relative optical densities of bands that are normalized to β-actin expression. c Mesenchymal markers, including N-cadherin, fibronectin and vimentin, were examined by western blot analysis. (d, e and f) The quantitative ratios are shown as relative optical densities of bands that are normalized to the expression of β-actin. Cells were incubated with TGF-β1 (5 ng/mL) and increasing concentrations of OFE (4 and 20 μg/mL) for 48 h. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs. control; ANOVA (LSD)