Fig. 4

TGF-β1-induced upregulation of Slug protein and mRNA expression was blocked by OFE. a TGF-β1-induced upregulation of FN1 mRNA expression was blocked by OFE. b Western blot analysis was performed to examine the expression of Slug and Snail after SKOV3 treatment, as indicated. c Protein levels were further confirmed by semi-quantitative densitometry analyses. d Real-time PCR analysis to measure the mRNA levels of SNAI1 and SNAI2 in SKOV3 cells. Data are presented as mean ± SD from three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs. control; ANOVA (LSD)