Fig. 4

Differentiation of BM granulocytes. BM smears collected from the sternum at different time points were used. We determined the proportion of BM bands and segmented cells in the total number of neutrophils, and used a microscope to identify the degree of differentiation of BM neutrophils. a BM smears observed under oil microscopy (600×) showed granulocyte differentiation on day 14. b Leukocyte differential counts were carried out on a minimum of 500 cells on BM smears stained with Giemsa. Cells of the granulocytic lineage were classified as immature granulocytes (myeloblasts, promyelocytes, myelocytes, and metamyelocytes), mature granulocytes (bands and segmented), eosinophils, and mononuclear cells. Data were expressed as means ± SD. Comparison of CTX + APS group and CTX + G-CSF group showed a significant difference (**:P < 0.01, *: P < 0.05). Comparison of CTX + APS group and CTX group showed a significant difference (^ ^: P < 0.01, ^:P < 0.05)