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Table 1 Steps for purification of GSLL

From: Lectin from green speckled lentil seeds (Lens culinaris) triggered apoptosis in nasopharyngeal carcinoma cell lines

Purification step Yield (mg)/80 g seeds Specific hemagglutinating activity (units/mg) Total hemagglutinating activity (105 units) Recovery of hemagglutinating activity (%) Fold of purification
Crude extract 4647 138 6.42 100 1
Affi-gel blue gel 580.9 768 4.46 69.4 5.57
Mono Q 124.9 1024 1.28 19.9 7.42
Superdex 75 11.8 6960 0.82 12.8 50.43