Fig. 7

Effects of QDP on LPS-induced IκB α degradation and p65 nuclear translocation. a Cells were pretreated with the indicated concentrations of QDP for 1 h and incubated with LPS (1 μg/mL) for another 30 min. Cells were lysed and IκB α expression was determined by Western blot. b Cells were pretreated with 1 μg/mL QDP for 1 h prior to stimulation with LPS (1 μg/mL) for 1 h. The nuclear localization of p65 was determined using fluorescence microscopy after staining with DAPI, anti-p65, and FITC-labeled anti-rabbit IgG antibody (p65 sequestered in the cytoplasm were indicated by yellow arrows and p65 translocated into nuclear were indicated by blue arrows). Data represent the mean ± SD (n = 3). The images shown are the representatives of three independent experiments