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Table 2 Characteristics of the DNA barcodes evaluated in this study

From: Identification of Hippophae species (Shaji) through DNA barcodes

DNA region ITS2 psbA-trnH ITS2 + psbA-trnH
Number of individuals 86 75 75
Number of species 7 7 7
PCR/sequencing success (%) 100/100 100/100 100/100
Amplified sequence length (bp) 221–223 300–313 521–530
Aligned sequence length (bp) 227 320 547
Average GC content (%) 52.72 25.62 37.18
Variable sites 43 19 59
Haplotypes 23 23 28
Intra-specific distance range (mean) 0–0.0571 (0.0041) 0–0.0340 (0.0021) 0–0.0297 (0.0025)
Inter-specific distance range (mean) 0–0.1298 (0.0594) 0–0.0489 (0.0237) 0.0019–0.0708 (0.0363)