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Table 2 Sequence characteristics of ITS2 for G. littoralis and its adulterants

From: Authentication of commercial processed Glehniae Radix (Beishashen) by DNA barcodes

Sequence characteristics

 

Amplification efficiency of G. littoralis

100 %

Sequencing efficiency of G. littoralis

100 %

Length of G. littoralis

228 bp

Amplification efficiency of all taxa

100 %

Sequencing efficiency of all taxa

100 %

Length of all taxa

202–266 bp

Aligned length

275 bp

GC content range in G. littoralis

58.6 %

GC content range in all taxa, (mean GC content in all taxa)

49.3–61.8 (56.0) %

Number (and %) of variable sites in all taxa

189 (68.7 %)

Intra-specific distance

0.000

Inter-specific distance (mean)

0.248–0.987 (0.468)