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Table 2 Sequence characteristics of ITS2 for G. littoralis and its adulterants

From: Authentication of commercial processed Glehniae Radix (Beishashen) by DNA barcodes

Sequence characteristics  
Amplification efficiency of G. littoralis 100 %
Sequencing efficiency of G. littoralis 100 %
Length of G. littoralis 228 bp
Amplification efficiency of all taxa 100 %
Sequencing efficiency of all taxa 100 %
Length of all taxa 202–266 bp
Aligned length 275 bp
GC content range in G. littoralis 58.6 %
GC content range in all taxa, (mean GC content in all taxa) 49.3–61.8 (56.0) %
Number (and %) of variable sites in all taxa 189 (68.7 %)
Intra-specific distance 0.000
Inter-specific distance (mean) 0.248–0.987 (0.468)