Table 2 Analysis of the five candidate barcode loci of Uncaria
From: Molecular identification of Uncaria (Gouteng) through DNA barcoding
Items | ITS2 | rbcL | psbA–trnH | ITS | matK |
|---|---|---|---|---|---|
Species numbers | 14 | 15 | 10 | 14 | 7 |
Accession no. | 77 | 63 | 49 | 58 | 10 |
Length range (average) (bp) | 210–221 (220) | 512–656 (608) | 235–315 (287) | 607–621 (616) | 757–814 (808) |
Average of GC content (%) | 66.3 | 43.0 | 24.8 | 62.8 | 33.1 |
No. of variable sites in all taxa | 41 | 16 | 173 | 86 | 13 |
No. of indels in all taxa | 2 | 0 | 39 | 14 | 0 |
BLAST method (identification efficiency [%]) | 87.0 | 42.9 | 95.9 | 91.4 | 80 |
Nearest distance method (identification efficiency [%]) | 92.2 | 76.2 | 95.9 | 84.5 | 80 |