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Fig. 5 | Chinese Medicine

Fig. 5

From: Novel cycloartane triterpenoid from Cimicifuga foetida (Sheng ma) induces mitochondrial apoptosis via inhibiting Raf/MEK/ERK pathway and Akt phosphorylation in human breast carcinoma MCF-7 cells

Fig. 5

Apoptosis induced by ADHC-AXpn in MCF-7 cells. a Representative image of MCF-7 cells stained with Hoechst 33258 after treatment with ADHC-AXpn for 48 h, under fluorescence microscope; red arrows: cell shrinkage, nuclear fragmentation. b Apoptosis quantification, using Annexin V/PI double staining assay after treatment with ADHC-AXpn for 36 h. MCF-7 cells were harvested and stained with PI and Annexin V-FITC in darkness for 15 min, followed by flow cytometry analysis. Lower right: Annexin V+/PI− (early apoptosis); upper right: Annexin V+/PI+ (late apoptosis). c Western blot showing PARP cleavage in MCF-7 cell lysates after treatment with ADHC-AXpn, using indicated antibodies. Loading control: β-actin; positive control: doxorubicin (Dox), 5 μM

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Chinese Medicine

ISSN: 1749-8546

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