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Fig. 5 | Chinese Medicine

Fig. 5

From: A novel coumarin, (+)-3′-angeloxyloxy-4′-keto-3′,4′-dihydroseselin, isolated from Bupleurum malconense (Chaihu) inhibited NF-κB activity

Fig. 5

Inhibitory effect of Pd-Ib on iNOS and COX-2 expression in LPS-stimulated RAW-Blue cells. After macrophages were treated with 1 μg/mL LPS in the absence or presence of various concentrations of Pd-Ib (5, 10 and 20 μg/mL) for 20 h, the protein levels of iNOS and COX-2 were determined by western blotting; β-actin was used as a loading control. a Representative western blotting images. b The protein levels of iNOS and COX-2 were calculated with ImageJ software. Data were derived from three independent experiments and presented as mean ± SD. #Compared with the control group. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. LPS-alone group

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