Fig. 6From: A novel coumarin, (+)-3′-angeloxyloxy-4′-keto-3′,4′-dihydroseselin, isolated from Bupleurum malconense (Chaihu) inhibited NF-κB activityEffect of pro-inflammatory factors expression on nitric oxide production in LPS-stimulated RAW-Blue cells. After macrophages were treated with 1 μg/mL LPS in the absence or presence of various concentrations of Pd-Ib (5, 10 and 20 μg/mL) for 20 h, the mRNA levels of iNos (a) Tnf-α (b) and Il-1β (c) were determined by real-time PCR, and the production of NO (d) was determined by Griess reagent. Data were derived from three independent experiments and presented as mean ± SD. con vehicle control group. #Compared with the control group. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. LPS-alone groupBack to article page