Fig. 2

Determination of the optimal number of control genes for normalization under different treatment conditions. If the V 2/V 3 value is less than 0.15, two genes are suitable for normalization as recommended by geNorm. Otherwise, more genes are needed until the V n /V n+1 value is less than 0.15, and the suitable gene number for normalization is n. a, b, c, d, and e Optimal number of control genes for normalization after IAA, ABA, 6-BA, MeJA, and GA treatment, respectively. f Optimal number of control genes for normalization under different temperatures