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Table 10 Ranking order of the reference genes under different temperatures (Better-Good-Average)

From: Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

Method

1

2

3

4

5

6

7

8

9

10

11

12

13

14

DeltaCT

RPB2

his3-2

RPB1-2

CYP

APT

β-Act

GAPDH

MAPK

RPB1-1

β-Tub

EIF

his3-1

RP

SAMDC

BestKeeper

EIF

β-Act

RPB2

RPB1-1

RPB1-2

β-Tub

his3-2

SAMDC

GAPDH

CYP

APT

MAPK

his3-1

RP

NormFinder

his3-2

RPB2

CYP

RPB1-2

APT

β-Act

GAPDH

MAPK

RPB1-1

β-Tub

EIF

his3-1

RP

SAMDC

geNorm

RPB1-2/RPB2

 

GAPDH

APT

CYP

his3-2

MAPK

β-Act

RPB1-1

EIF

β-Tub

his3-1

RP

SAMDC

Recommended comprehensive ranking

RPB2

RPB1-2

his3-2

β-Act

CYP

APT

EIF

GAPDH

RPB1-1

MAPK

β-Tub

SAMDC

his3-1

RP