Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

Table 3 The average C_t value (mean ± SD) of each candidate gene under different conditions

	GAPDH	MAPK	β-Act	RPB2	RPB1-1	RPB1-2	his3-1	his3-2	APT	SAMDC	RP	β-Tub	EIF	CYP
IAA	22.88 ± 1.26	29.49 ± 0.94	30.13 ± 0.83	29.60 ± 1.30	31.31 ± 1.19	32.10 ± 0.97	26.49 ± 1.06	25.53 ± 1.43	29.11 ± 1.05	32.57 ± 0.97	28.21 ± 0.80	27.98 ± 0.89	28.57 ± 0.96	22.45 ± 0.97
ABA	23.69 ± 0.73	30.76 ± 0.74	30.66 ± 1.50	29.71 ± 2.03	31.58 ± 1.34	31.83 ± 1.54	27.04 ± 0.82	26.22 ± 0.62	29.43 ± 0.88	32.44 ± 0.87	28.39 ± 0.70	28.18 ± 1.05	28.92 ± 0.66	23.35 ± 0.48
6-BA	22.66 ± 1.23	27.48 ± 0.81	31.00 ± 0.87	29.81 ± 1.06	31.57 ± 1.01	32.51 ± 1.27	26.53 ± 0.68	22.37 ± 0.92	32.06 ± 1.01	28.40 ± 1.19	27.96 ± 0.69	24.12 ± 1.05	24.51 ± 1.09	23.26 ± 0.74
MeJA	23.91 ± 1.23	30.85 ± 1.18	29.97 ± 0.78	32.45 ± 1.00	30.69 ± 0.81	32.86 ± 0.86	26.91 ± 0.91	26.73 ± 0.86	31.65 ± 0.95	33.24 ± 1.18	28.20 ± 1.15	28.13 ± 1.23	29.95 ± 0.92	23.53 ± 0.67
GA	24.33 ± 1.32	31.47 ± 0.61	30.36 ± 1.28	31.82 ± 1.26	30.60 ± 1.20	32.06 ± 0.96	26.52 ± 1.23	25.66 ± 1.27	31.48 ± 1.34	32.58 ± 1.22	27.48 ± 1.61	27.72 ± 1.64	29.22 ± 1.53	23.48 ± 0.96
Temperature	23.16 ± 1.01	27.08 ± 1.18	30.37 ± 0.68	29.94 ± 0.88	31.50 ± 0.93	31.90 ± 0.94	25.87 ± 1.56	22.30 ± 0.77	32.12 ± 1.08	28.64 ± 1.17	27.37 ± 1.77	24.27 ± 1.11	25.08 ± 1.04	22.80 ± 0.93

ISSN: 1749-8546