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Table 1 Transitions and collision energies used in LC-MS/MS for the detection of the probe substrates, metabolites and the internal standard

From: Kinetics of cytochrome P450 enzymes for metabolism of sodium tanshinone IIA sulfonate in vitro

Compound name Ion mode Precursor ion (m/z) Product ion (m/z) Collision energy (eV)
Sodium tanshinone IIA sulfonate Negative 373 358 20
Acetaminophen Positive 152 110 15
7-hydroxyl coumarin Positive 162.9 107.0 20
1-hydroxyl midazolam Positive 342 324 20
4-hydroxyl tolbutamide Positive 287.0 171.0 15
4-hydroxyl mephenytoin Positive 235.0 150.0 10
α-hydroxyl metoprolol Positive 284.3 116.0 20
6-hydroxyl chlorzoxazone Positive 195 138 20
Irbesartan (IS) Positive 429.0 206.9 22