Table 1 Transitions and collision energies used in LC-MS/MS for the detection of the probe substrates, metabolites and the internal standard
From: Kinetics of cytochrome P450 enzymes for metabolism of sodium tanshinone IIA sulfonate in vitro
Compound name | Ion mode | Precursor ion (m/z) | Product ion (m/z) | Collision energy (eV) |
|---|---|---|---|---|
Sodium tanshinone IIA sulfonate | Negative | 373 | 358 | 20 |
Acetaminophen | Positive | 152 | 110 | 15 |
7-hydroxyl coumarin | Positive | 162.9 | 107.0 | 20 |
1-hydroxyl midazolam | Positive | 342 | 324 | 20 |
4-hydroxyl tolbutamide | Positive | 287.0 | 171.0 | 15 |
4-hydroxyl mephenytoin | Positive | 235.0 | 150.0 | 10 |
α-hydroxyl metoprolol | Positive | 284.3 | 116.0 | 20 |
6-hydroxyl chlorzoxazone | Positive | 195 | 138 | 20 |
Irbesartan (IS) | Positive | 429.0 | 206.9 | 22 |