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Table 2 The sequence of the forward primer and reverse primer used for FQ-PCR assays

From: Herb-partitioned moxibustion upregulated the expression of colonic epithelial tight junction-related proteins in Crohn’s disease model rats

Name Primer sense Primer sequence (5′ → 3′) Amplification product (bp)
GAPDH Forward 5′-CCGAGGGCCCACTAAAGG-3′ 116
Reverse 5′-GCTGTTGAAGTCACAGGAGACAA-3′
Occludin Forward 5′-GGGACAGAGCCTATGGAA-3′ 191
Reverse 5′-GGAAGCGATGAAGCAAAA-3′
Claudin-1 Forward 5′-CTGGCTTCGCTGGGATGGA -3′ 100
Reverse 5′-TGGCCTGAGCAGTCACGATGTT-3′
ZO-1 Forward 5′-GATGAGCGGGCTACCTTA-3′ 126
Reverse 5′-ATGGGAGCGAACTGAATG-3′