Table 1 Primer sequences used for qPCR amplification
From: Loki zupa (Luooukezupa) decoction reduced airway inflammation in an OVA-induced asthma mouse model
Gene | Primer sequence |
|---|---|
IL-4 (NM_021283.2) | Forward primer: 5′-CAGCAACGAAGAACACCACA-3′ Reverse primer: 5′-AATCCAGGCATCGAAAAGCC-3′ |
IL-5 (NM_010558.1) | Forward primer: 5′-CAGTGTGAATGAGAGCCAGC-3′ Reverse primer: 5′-TTCTGTTGGCATGGGGTAGT-3′ |
IL-13 (NM_008355.3) | Forward primer: 5′-TTGAGGAGCTGAGCAACA-3′ Reverse primer: 5′-CCATGCTGCCGTTGCA-3′ |
IFN-γ (NM_008337.3) | Forward primer: 5′-AGCTCTTCCTCATGGCTGTT-3′ Reverse primer: 5′-TCCTTTTGCCAGTTCCTCCA-3′ |
GAPDH (NM_008084.2) | Forward primer: 5′-GTGGAGTCTACTGGTGTCTTC-3′ Reverse primer: 5′-CTCAGTGTCCCACCACCCT-3′ |