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Table 2 Antiproliferation of P. kesiya extract and melphalan combination in U937, HepG2, and Vero cell lines

From: Synergistic effects of melphalan and Pinus kesiya Royle ex Gordon (Simaosong) extracts on apoptosis induction in human cancer cells

IC50 Ratio

% Antiproliferation

P. kesiya

Melphalan

U937

HepG2

Vero

0

1

50

50

50a

1

0

50

50

Inactiveb

1

1

100 ± 7.0

95.7 ± 12.2

6.8 ± 1.9

0.02

1

46.0 ± 5.7

Nd

Nd

0.05

1

59.8 ± 5.3

Nd

Nd

0.1

1

76.6 ± 13.5

Nd

1.0 ± 3.5

0.2

1

79.3 ± 3.7

43.7 ± 2.9

4.0 ± 6.1

0.5

1

100 ± 7.0

82.1 ± 4.9

7.3 ± 1.9

1.5

1

100 ± 2.2

93.4 ± 1.6

16.4 ± 4.7

2

1

100 ± 11.3

100 ± 3.1

24.7 ± 9.1

1

0.05

46.8 ± 9.5

34.2 ± 4.4

Nd

1

0.1

51.8 ± 5.5

46.0 ± 9.1

Nd

1

0.2

73.0 ± 4.2

60.7 ± 16.4

0 ± 3.3

1

0.5

76.4 ± 3.6

65.1 ± 9.1

1.8 ± 1.2

1

1.5

100 ± 2.0

100 ± 10.5

8.6 ± 1.4

1

2

100 ± 3.4

100 ± 4.9

9.5 ± 1.9

  1. Two-way ANOVA detected significant antiproliferative activity differences among various IC50 ratios (P < 0.001) and among various cell lines (P < 0.001). A significant two-way interaction was observed between IC50 ratio and various cell lines (P < 0.001)
  2. Nd not determined
  3. aIC50 value of melphalan in Vero cells equals 59.9 ± 3.2 µg/mL
  4. bInactive when IC50 of P. kesiya in Vero cells was >500 µg/mL