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Fig. 3 | Chinese Medicine

Fig. 3

From: Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip

Fig. 3

Herbal extracts in NGF-induced pNF-68/200 expression in PC12 cells. a PC12 cells were transfected with pNF-68/200-Luc and serum-starved for 3 h before co-treatment of 50 ng/mL of NGF and extract at 0.1 mg/mL. Cell lysates (50 μg) were subjected to a luciferase assay. b Activation by NGF is dose-dependent. K252a (100 nM, positive control) suppressed the NGF-induced response (left inset). Data are expressed as a percentage of change (mean ± SD, n = 4), where the values of NGF-added culture was set as 0. Statistical comparisons are between NGF alone (or its corresponding control) and the co-treatments, *P < 0.05, **P < 0.01, ***P < 0.001

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