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Fig. 4 | Chinese Medicine

Fig. 4

From: Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip

Fig. 4

Herbal extracts in NGF-induced CREB phosphorylation in PC12 cells. PC12 cells were serum-starved for 3 h before co-treatment of 50 ng/mL of NGF and extract at 0.1 mg/mL, or K252a at 100 nM. Cell lysates (20 μg) were subjected to western blotting for phosphorylation analysis. NGF induced phosphorylation of CREB (P-CREB at ~42 kDa) in a time-dependent manner, which was altered by treatment of herbal extracts (inset). Band intensity was qualified. Data are expressed as a percentage of change (mean ± SD, n = 4), where the values of NGF added culture was set as 0. Statistical comparisons are between the NGF alone and the co-treatments: *P < 0.05, **P < 0.01, ***P < 0.001

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