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Fig. 4 | Chinese Medicine

Fig. 4

From: Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase

Fig. 4

Vimentin immunohistochemistry. One (b) or 7 days following the sham procedure (k; Group 1), the Müller cells were demonstrated with vimentin (green) immunolabeling at the end feet (arrow heads) in the ganglion cell layer (GCL) and at the processes in the IPL inner plexiform layer (arrows), INL inner nuclear layer and ONL outer nuclear layer. In contrast with the Group 1, anti-vimentin immunolabeling was increased one or 7 days after ischemia plus reperfusion (I/R) with preadministration of vehicle [Group 2; on I/R day 1, (c); on I/R day 7, (l)]. This enhancement was attenuated by pretreatment with 2.8 g/kg/day [Group 3; on I/R day 1, (d) on I/R day 7, (m)] or 4.2 g/Kg/day of CJDHW [Group 4; on I/R day 1, (e); on I/R day 7, (n)]. The cellular nuclei of the Group 1 (a, j) were immunolabeled with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; blue). GFAP immunohistochemistry. In contrast with the Group 1, one (f) or 7 days following the sham procedure (o), anti-GFAP immunolabeling was also increased 1 (g) or 7 days day after ischemia plus reperfusion (I/R) with preadministered vehicle (p, Group 2). This enhancement was blunted by pretreatment with 2.8 g/kg/day (Group 3; on I/R day 1, H; on I/R day 7, q) or 4.2 g/kg/day of CJDHW (Group 4; on I/R day 1, I; on I/R day 7, r). GFAP, glial fibrillary acidic protein; CJDHW Chi-Ju-Di-Huang-Wan. Scale bar = 25 μm

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