Fig. 8

Gel zymography. An example of a gelatin zymogram (a) used for the quantification of matrix metalloproteinase-9 (MMP-9) quantitation protein expression by densitometry. Gelatinolytic bands of 72 and 97 kDa corresponded to MMP-2 and active MMP-9, respectively. Lane 1, the sham procedure retina (Group 1); Lane 2, the vehicle-pretreated ischemic retina (I/R + Vehicle; Group 2); Lane 3, the SB203580 (p38 MAPK inhibitor)-pretreated ischemic retina (I/R + SB203580; Group 5); Lanes 4 and 5, the ischemic retina respectively pretreated with 4.2 g/kg/day (I/R + CJDHW4.2; Group 4) and 2.8 g/kg/day of CJDHW (I/R + CJDHW2.8; Group 3). Each bar (b) indicates the mean ± SD (B; n = 4–5). **indicates significance (P < 0.01; Group 1 vs. 2). ^††indicates significance (P < 0.01; Group 2 vs. 4; Group 2 vs. 5). †indicates significance (P < 0.05; Group 2 vs. 3). In contrast to the results for MMP-9, the expression of MMP-2, when measured at 24 h after ischemia and pretreatment with vehicle (Group 2) or CJDHW4.2 (Group 4)/CJDHW2.8 (Group 3), was found to have a similar protein expression level to that of the control Group (Sham; Group 1)