Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase

Table 2 Hematoxylin and eosin staining

Group	1	2	3	4
Retinal thickness on day 1
Whole	174.12 ± 3.64	165.43 ± 4.44**	168.45 ± 12.89	172.00 ± 1.57^†
INL	41.68 ± 2.69	37.13 ± 5.04*	37.52 ± 2.09	39.74 ± 3.13^†
IPL	41.93 ± 1.58	36.81 ± 2.21**	37.16 ± 4.55	40.39 ± 0.86^†
Retinal thickness on day 7
Whole	180.25 ± 4.35	141.02 ± 9.47**	156.18 ± 7.92^†	171.78 ± 10.38^††
INL	38.10 ± 1.39	24.74 ± 3.60**	29.23 ± 5.43	34.45 ± 4.02^†
IPL	38.38 ± 1.43	20.34 ± 1.79**	26.20 ± 3.60^†	33.53 ± 1.85^†

In comparison of the control retina, 1 or 7 days following sham procedure (Group 1; Sham 1 day, n = 6; Sham 7 days, n = 6), after retinal ischemia plus 1 or 7 days of reperfusion and pretreatment with vehicle (Group 2; I/R 1 day + vehicle, n = 4; I/R 7 days + vehicle, n = 5), there was a significant decrease (* P < 0.05; ** P < 0.01) in the thickness of the whole retina, the INL and the IPL. In contrast, this significant decrease was dose-dependently (with a less effect at 2.8 g/kg/day, I/R + CJDHW2.8, Group 3, n = 4) and significantly (^† P < 0.05; ^†† P < 0.01; Group 3; I/R + CJDHW4.2, Group 4, n = 4) inhibited by pretreatment with CJDHW. The results are the mean ± SD (μm)
CJDHW4.2 Chi-Ju-Di-Huang-Wan at 4.2 g/kg/day, INL inner nuclear layer, IPL inner plexiform layer

ISSN: 1749-8546