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Table 8 Gel zymography

From: Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase

Group (n = 4–5)

1

2

3

4

5

MMP-9/β-actin

1.00 ± 0.00

5.03 ± 1.57**

3.05 ± 0.88†

1.35 ± 0.41††

1.49 ± 0.47††

  1. The ratios of the protein expression levels of MMP-9 relative to that of β-actin
  2. This study included 5 Groups, namely, Group 1 (the sham procedure retina) as well as Groups 2, 3, 4 and 5 [(the retina subjected to ischemia plus reperfusion respectively pretreated with vehicle (I/R + vehicle), 2.8 g/kg/day of CJDHW (I/R + CJDHW2.8), 4.2 g/kg/day of CJDHW (I/R + CJDHW4.2) and SB203580 (p38 MAPK inhibitor)]
  3. CJDHW Chi-Ju-Di-Huang-Wan
  4. ** indicates significance (P < 0.01; Group 1 vs. 2)
  5. †† indicates significance (P < 0.01; Group 2 vs. 4; Group 2 vs. 5). †indicates significance (P < 0.05; Group 2 vs. 3). The results are mean ± SD (n = 4–5)
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Chinese Medicine

ISSN: 1749-8546

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