Table 2 Results of methodology validation in terms of linear regression, LOD, LOQ, repeatability, accuracy, and stability
Analyte | Linearity | LOD (ng/mL) | LOQ (ng/mL) | Repeatability (RSD, %, n = 6) | Spike recovery (RSD, %, n = 3) | Stability (RSD, %, n = 6) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Range (ng/mL) | Equation | r2 | Intra-day | Inter-day | High | Middle | Low | ||||
1 | 50.60–404.80 | Y = 148.2X − 704.4 | 0.9996 | 13.67 | 45.58 | 3.45 | 4.81 | 96.37 (2.08) | 96.55 (0.18) | 105.99 (2.65) | 3.16 |
2 | 397.50–3180.00 | Y = 33.8X − 721.8 | 0.9989 | 104.61 | 348.68 | 2.93 | 4.84 | 98.86 (2.16) | 98.67 (0.65) | 94.99 (2.00) | 2.46 |
3 | 329.10–10530.00 | Y = 56.9X + 10454.0 | 0.9997 | 43.38 | 144.60 | 1.20 | 3.37 | 101.88 (2.21) | 101.73 (0.65) | 94.37 (1.72) | 1.81 |
4 | 3187.50–25500.00 | Y = 7.3X + 2059.8 | 0.9997 | 233.80 | 779.34 | 2.53 | 5.54 | 96.96 (2.22) | 104.26 (0.78) | 102.12 (5.48) | 4.59 |
5 | 1659.40–53100.00 | Y = 16.9X + 18376.0 | 0.9989 | 174.67 | 582.64 | 4.06 | 4.64 | 102.71 (1.99) | 96.39 (0.31) | 102.31 (1.94) | 2.59 |
6 | 59.48–237.93 | Y = 1203.4X + 9810.8 | 0.9992 | 11.65 | 38.84 | 3.78 | 4.66 | 101.38 (5.10) | 104.00 (0.69) | 104.50 (1.23) | 3.98 |
7 | 126.28–5387.76 | Y = 493.9X + 11718.0 | 0.9999 | 25.25 | 84.18 | 2.58 | 4.17 | 97.14 (2.19) | 95.46 (1.27) | 95.10 (0.37) | 2.58 |
8 | 116.50–1864.00 | Y = 170.7X + 5289.2 | 0.9996 | 17.83 | 59.44 | 1.05 | 4.54 | 96.77 (0.99) | 101.81 (0.77) | 104.52 (0.51) | 1.56 |
9 | 13.02–208.33 | Y = 378.9X + 1346.2 | 0.9994 | 2.77 | 9.24 | 1.55 | 3.66 | 101.45 (4.45) | 97.26 (1.42) | 104.54 (2.29) | 1.29 |
10 | 13.27–106.12 | Y = 12974.0X − 33626.0 | 0.9994 | 2.14 | 7.13 | 1.19 | 4.71 | 101.76 (2.68) | 103.99 (1.79) | 105.29 (1.15) | 1.51 |
11 | 52.95–211.80 | Y = 592.1X + 6742.6 | 0.9993 | 9.57 | 31.05 | 2.42 | 3.97 | 102.07 (1.53) | 95.27 (0.78) | 105.62 (0.58) | 2.37 |
12 | 1273.44–81500.00 | Y = 44.0X + 62221.0 | 0.9996 | 49.97 | 166.57 | 2.50 | 4.59 | 100.78 (2.65) | 98.85 (3.36) | 101.55 (3.70) | 4.67 |
13 | 33.04–198.21 | Y = 2767.1X − 52144.0 | 0.9988 | 7.62 | 25.41 | 3.46 | 4.43 | 95.89 (0.71) | 99.58 (3.47) | 94.63 (0.77) | 2.85 |
14 | 204.10–13062.50 | Y = 132.0X + 17621.0 | 0.9992 | 28.98 | 96.62 | 1.69 | 4.89 | 97.72 (3.65) | 98.26 (0.72) | 99.54 (3.98) | 4.28 |
15 | 259.38–4150.00 | Y = 47.4X − 1173.4 | 0.9989 | 71.38 | 237.95 | 2.64 | 3.89 | 102.64 (2.18) | 99.14 (0.64) | 106.11 (1.39) | 2.29 |
16 | 1767.19–28275.00 | Y = 117.5X + 127121.0 | 0.9989 | 26.61 | 88.71 | 1.89 | 4.29 | 97.29 (2.79) | 98.27 (0.29) | 96.19 (2.40) | 1.87 |
17 | 118.64–2548.47 | Y = 1291.1X − 78900.0 | 0.9998 | 27.23 | 90.76 | 1.07 | 1.69 | 96.64 (1.39) | 101.78 (0.77) | 94.88 (1.05) | 1.73 |