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Fig. 3 | Chinese Medicine

Fig. 3

From: Inhibitory effect of trans-ferulic acid on proliferation and migration of human lung cancer cells accompanied with increased endogenous reactive oxygen species and β-catenin instability

Fig. 3

The long-term effect of trans-FA on colony formation and anchorage-independent growth assay of NSCLC. a The assessment of colony formation, a 2D indicator of cell proliferation. Adherent H1299 cells were treated with indicated concentrations for 24 h. 5 × 10 cells treated with trans-FA were allowed to form colonies for 11 days. Afterward, colonies were fixed and stained using crystal violet. c Representative results showed the formation of tumor spheres, an indicator of 3D for anchorage-independent growth. H1299 cells were treated with indicated concentrations of trans-FA for 13 days using the soft agar assay. b, d Quantitative analysis of results from a and c showed the number and size of tumorspheres. *P < 0.05 and **P < 0.001, respectively

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Chinese Medicine

ISSN: 1749-8546

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