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Table 3 Linearity, precision, repeatability, recovery, MDL, and LOQ of the established method

From: Quality control of Lycium chinense and Lycium barbarum cortex (Digupi) by HPLC using kukoamines as markers

Parameter

Kukoamine A

Kukoamine B

Linearity (12 points)

3.91–250.00 mg/L; r2 = 0.9999a

4.12–263.50 mg/L; r2 = 0.9999a

Precision (RSD)b, %

1.29

0.57

Repeatability (RSD)c, %

1.81

0.92

Mean recovery (n = 5)d

95.89%

90.03–102.30%

100.32%

98.49–101.67%

MDL (µg/mL)

1.76

1.86

LOQ (µg/mL)

8.78

9.29

  1. MDL method detection limit, LOQ limit of quantification
  2. ar2, square of correlation coefficient for the linear regression (P < 0.05)
  3. bPrecision was tested based on six injections of a standard solution with 62.50 µg/mL kukoamine A and 131.75 µg/mL kukoamine B. RSD (%) = 100 × SD/mean
  4. cRepeatability was tested on five replicate samples
  5. dRecovery (%) = 100 × (amount found − original amount)/amount spiked. The results were presented as the mean recovery and recovery range. The amounts of spiked components for the recovery test were 10.4, 20.8, and 41.6 mg for KB, and 1.5, 3.0, and 4.5 mg for KA
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Chinese Medicine

ISSN: 1749-8546

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