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Fig. 1 | Chinese Medicine

Fig. 1

From: Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase

Fig. 1

GTE blocks T cell activation. a CD69 induction decreased in GTE-treated Jurkat T cells. Jurkat T cells were activated by PMA/ionomycin alone (P/I; solid line) or with 25 μg/mL GTE (long dashed line). GTE treatment alone (short dashed line) was used as a control. The cells were stained with an anti-CD69-PE antibody. The CD69-positive cells were then analyzed by flow cytometry. Data were assessed with FlowJo software. b Decreased proliferation of GTE-treated PBMCs. Freshly purified PBMCs were pretreated with various doses of GTE for 30 min and then stimulated by 2.5 μg/mL PHA. Cell proliferation was examined by 3H-thymidine incorporation after a 20 h pulse with 0.5 μCi/well 3H-thymidine. Data are expressed as counts per minute (CPM) of 3H-thymidine uptake. A significant difference from the vehicle is indicated as *P < 0.05 or **P < 0.01

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