Fig. 4

GTE attenuates JNK and p38 activation. a Jurkat T cells were activated by PHA alone or with GTE. Protein extracts were prepared at the indicated time points and subjected to western blot analysis. Activation of ERK, JNK and p38 was measured by phosphorylation of ERK, JNK, and p38. ERK2, JNK1, and p38 served as internal controls. Densitometric quantification of fold inductions of the levels of b p-ERK2 relative to ERK2, c p-p38 relative to p38, and d p-JNK relative to JNK1 protein. The densities of protein bands were determined by Image Lab software. Densitometric quantification of each protein was normalized by the internal control. The level of p-ERK2 was normalized by ERK2. The level of p-p38 was normalized by p38, and the level of p-JNK was normalized by JNK1. Data represented fold activation from the non-activated (0 h) density (b–d). A significant difference from the control is indicated as *P < 0.05