Fig. 5

GTE blocks prolonged JNK-induced AICD. a Jurkat cells were treated with GTE, sodium orthovanadate (V; 1.5 mM), and/or PMA/ionomycin (P/I; 50/80 ng/mL) for 24 h. The cells were collected and stained with PI in a hypotonic solution. Apoptotic cells were analyzed by flow cytometry. Data were assessed with FlowJo software. b CD4 T cells were isolated from mouse spleen, activated with anti-CD3/CD28 antibodies, and cultured in the presence of IL-2. The activated T cells were re-activated by 0.5 μg/mL anti-CD3 antibody at day 5 and then harvested for an apoptosis assay. The left panel is the original apoptosis data. The right panel is quantitative data. The extent of the sub-G0/G1 peak representing apoptotic cells was measured by gating on cells in the region below the G0/G1 peak. A significant difference from the control is indicated as **P < 0.01