Table 2 Nucleotide sequences of the primers used for real-time PCR
Forward nucleotide sequence 5′–3′ | Reverse nucleotide sequence 5′–3′ | |
|---|---|---|
IRS-1 | GGCAGCAATGAGGGCAACT | TGGAAGCTGATGCTGGCATA |
IL-6 | GTTGCCTTCTTGGGACTGAT | GCCATTGCACAACTCTTTTCT |
TNF-α | GCCATTGCACAACTCTTTTCT | AGTTGGTCCCCCTTCTCC |
ICAM-1 | GCTTCACACTTCACAGTTACTT | AGAGGACCTTAACAGTCTACAAC |
PI3K | CGGCGTGACATGTAGGCTCTCA | ACGGCCCGCACTGTAACCTAT |
Akt | TGTGACCATGAACGAGTTTGA | GTCGTGGGTCTGGAATGAGTA |
IKKβ | TGCTGCCCAAGGTAGAAGAGGTAG | AGGCACTGGAAGGCTGGGACATTA |
NF-κB | CACCTCACCGGCCTCATCCACA | AGTCCCCGCGCTGCTCCTCTAT |
GAPDH | GGTGGAGCCAAAAGGGTCAT | CGTGGTTCACACCCATCACA |