Skip to main content

Advertisement

Table 1 tR (min) of four saponins in Paridis on different columns

From: A simple method for HPLC retention time prediction: linear calibration using two reference substances

No. Brand Chonglou saponin VII Chonglou saponin VI Chonglou saponin II Chonglou saponin I
col1 Discovery C 18 a 21.234 ± 0.021 23.001 ± 0.006 32.773 ± 0.007 35.118 ± 0.004
col2 Discovery C 18 b 16.555 ± 0.010 17.989 ± 0.006 27.161 ± 0.011 29.154 ± 0.002
col3 Xbridge C 18 a 21.101 ± 0.004 23.070 ± 0.027 32.483 ± 0.021 34.963 ± 0.006
col4 BDS HypersilC 18 a 21.014 ± 0.011 22.898 ± 0.018 32.679 ± 0.032 35.170 ± 0.036
col5 Inertsil ODS-2a 22.132 ± 0.009 24.502 ± 0.004 33.176 ± 0.017 35.936 ± 0.003
col6 Kromasil C 18 a 21.276 ± 0.012 23.693 ± 0.016 32.618 ± 0.006 35.929 ± 0.007
col7 Luna C18(2)a 20.760 ± 0.018 23.362 ± 0.008 30.941 ± 0.011 33.813 ± 0.003
col8 Luna C18(2)b 16.551 ± 0.008 18.865 ± 0.023 25.923 ± 0.016 28.310 ± 0.011
col9 Inertsil ODS-3b 17.225 ± 0.006 19.640 ± 0.005 26.754 ± 0.013 29.471 ± 0.005
col10 Alltima C 18 a 20.856 ± 0.008 23.752 ± 0.021 31.687 ± 0.011 34.872 ± 0.004
col11 Symmetry C 18 a 21.016 ± 0.015 22.076 ± 0.016 32.470 ± 0.023 35.476 ± 0.010
col12 Gemini C 18 a 21.300 ± 0.014 23.756 ± 0.007 31.599 ± 0.003 34.337 ± 0.015
col13 CapcellpakC18MGa 21.076 ± 0.012 23.828 ± 0.004 31.627 ± 0.006 34.695 ± 0.001
col14 Zorbax Extend-C 18 a 17.201 ± 0.022 19.731 ± 0.003 27.525 ± 0.009 30.504 ± 0.005
col15 Sunfire C 18 a 21.652 ± 0.013 24.065 ± 0.024 32.375 ± 0.010 35.197 ± 0.006
col16 Sunfire C 18 b 17.501 ± 0.006 19.571 ± 0.018 27.401 ± 0.004 29.826 ± 0.010
col17 Nucleosil C18 HDa 21.452 ± 0.013 23.735 ± 0.006 32.747 ± 0.019 35.513 ± 0.011
col18 ODS Hypersila 19.669 ± 0.010 21.583 ± 0.022 30.361 ± 0.007 32.657 ± 0.005
col19 CapcellpakC18AQa 20.092 ± 0.013 22.560 ± 0.010 29.568 ± 0.006 32.218 ± 0.005
col20 Spherisorb ODS2a 18.684 ± 0.006 21.334 ± 0.021 28.580 ± 0.008 31.385 ± 0.004
col21 Zorbax SB-C 18 a 18.438 ± 0.015 20.986 ± 0.010 28.085 ± 0.008 30.849 ± 0.007
col22 DiamonsilC 18 a 22.082 ± 0.021 25.078 ± 0.005 32.475 ± 0.020 35.721 ± 0.023
col23 DiamonsilC 18 b 16.769 ± 0.007 19.154 ± 0.008 26.240 ± 0.011 28.935 ± 0.004
col24 Diamonsil C18(2)a 19.296 ± 0.016 22.819 ± 0.004 31.192 ± 0.019 34.710 ± 0.009
col25 Kromasil Eternity C 18 a 19.732 ± 0.017 22.099 ± 0.018 29.535 ± 0.004 32.153 ± 0.021
col26 Shim-pack VP-ODSb 19.015 ± 0.007 21.059 ± 0.014 29.401 ± 0.002 31.845 ± 0.001
col27 Agilent HC-C 18 a 23.492 ± 0.247 25.839 ± 0.250 34.884 ± 0.344 37.637 ± 0.330
col28 Agilent TC-C 18 a 20.609 ± 0.003 22.574 ± 0.005 30.806 ± 0.003 32.783 ± 0.002
col29 Venusil MP C 18 b 17.970 ± 0.007 20.468 ± 0.011 27.287 ± 0.031 29.985 ± 0.007
col30 Nucleosil C18 ABa 18.348 ± 0.012 20.209 ± 0.017 29.233 ± 0.011 31.893 ± 0.014
  StR (Average tR) 19.803 22.110 30.319 33.035
  1. a4.6 mm × 250 mm × 5 μm
  2. b4.6 mm × 150 mm × 5 μm