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Table 5 Comparison of prediction result in four methods (Paridis)

From: A simple method for HPLC retention time prediction: linear calibration using two reference substances

Method Maximum of ΔtR/min Average of ΔtR/min Number of positive columnsa
Unadjusted RR 1.811 0.420 12
Adjusted RR 1.562 0.410 9
Prediction by two points 0.836 0.283 25
Validation by multiple points 0.545 0.204 30
  1. atRL = 0.5 min