A simple method for HPLC retention time prediction: linear calibration using two reference substances

Chinese Medicine

Table 5 Comparison of prediction result in four methods (Paridis)

Method	Maximum of Δt_R/min	Average of Δt_R/min	Number of positive columns^a
Unadjusted RR	1.811	0.420	12
Adjusted RR	1.562	0.410	9
Prediction by two points	0.836	0.283	25
Validation by multiple points	0.545	0.204	30

ISSN: 1749-8546