Fig. 8

HS7 induces CDK inhibitors and inhibits phosphorylation of pRb protein in CL1-0 lung cancer cells after 72 h of treatment. HS7 dose-dependently increased protein levels of p15, p21 and p27, and decreased phosphorylated pRb protein (p-pRb). Cell lysates were analyzed by Western blot, using tubulin or GAPDH as loading control