Fig. 4

Immunohistological detection of ZO-1, occludin and F-actin protein levels in intestinal mucosa. A Immunohistological detection ZO-1 protein in the intestinal mucosa of animals from control group (a), WAS group (b), SGD group (c) and Dicetel group (d), (×400); average optical density levels of ZO-1 protein in the different groups (right panel). B Immunohistological detection of occludin protein levels in intestinal mucosa of animals from control group (a), WAS group (b), SGD group (c) and Dicetel group (d), (×400); average optical density levels of occludin in different groups (right panel). C Immunohistological detection of F-actin protein level in intestinal mucosa of animals from control group (a), WAS group (b), SGD group (c) and Dicetel group (d), (×400); average optical density levels of F-actin in different groups (right panel). Data were expressed as the mean ± SD. ^aP < 0.05 compared to the control group; ^bP < 0.05 compared to the WAS group; ^cP > 0.05 compared to the SGD group. n = 10 per group