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Fig. 3 | Chinese Medicine

Fig. 3

From: Ginsenoside G-Rh2 synergizes with SMI-4a in anti-melanoma activity through autophagic cell death

Fig. 3

SMI-4a induced autophagy via inhibiting AKT/mTOR signaling. a The autophagic body formation was tested by flow cytometry analysis through staining with acridine orange, after SMI-4a treatment for 48 h. b Cells were treated with various doses of SMI-4a for 24 h. The protein expression of Atg5, Beclin1 and LC3-II were evaluated by a western blotting assay. c The levels of total AKT (AKT), phosphorylated AKT (P-AKT), Total mTOR (mTOR), phosphorylated mTOR (P-mTOR) were tested by western blot. The results are expressed as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001

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