Table 1 Sequences of oligonucleotide primers and products size of RT-qPCR
mRNA | Primers (5′ → 3′) F: Forward R: Reverse | Product size (bp) |
|---|---|---|
Wnt1 | F: TGTTGACGGATTCCAAGAGT R: GAAGTAGACGAGGTCGTGAG | 235 |
β-catenin | F: GGCAACCCTGAGGAAGAAGA R: AGCGTCAAACTGCGTGGAT | 192 |
DKK1 | F: ATCAATTCCAACGCGATCAA R: GGTCAGAGGGCATGCATATT | 325 |
GSK3β | F: CCTGGTGCTGGACTATGT R: CAAGAGGTTCTGTGGTTTAA | 214 |
TCF4 | F: ACCACATGACTAGCAGGGATCT R: GGAGGAACTTTTCGGACTTTCT | 298 |
VEGF | F: TTACTGCTGTACCTCCACC R: ACAGGACGGCTTGAAGATG | 189 |
cyclin D1 | F: GAGAAGTTGTGCATCTACAC R: GAAGGGCTTCAACTGTTCC | 375 |
Survivin | F: GCTCTATGTAAGATCGCTTC R: CGTGAATCGCCATAATTATCC | 389 |
CTGF | F: GTGTGTGACGAGCCCAAGGA R: AGTTGGGTCTGGGCCAAATGT | 112 |
Fn1 | F: ACCATACCTGCCGAATGTAG R: CTCCTCTCCAATGGCGTAAT | 439 |
Collagen I | F: TGTTCGTGGTTCTCAGGGTAG R: TTGTCGTAGCAGGGTTCTTTC | 245 |
Collagen III | F: TGCCCACAGCCTTCTACACCT R: CAGCCATTCCTCCCACTCCAG | 254 |
GAPDH | F: TGGGTGTGAACCACGAGAA R: GGCATGGACTGTGGTCATGA | 143 |