Effective authentication of Placenta Hominis

Chinese Medicine

Table 2 Primers for amplification and sequencing

Species specificity	Primer name	Sequence (5′–3′)	Amplicon size (bp)	Annealing temp. (°C)
Homo sapiens	COI_human_F	GCAACCTTCTAGGTAACGACCAC	120	63
Homo sapiens	COI_human_R	GGGGAACTAGTCAGTTGCCAAAG	120	63
Bos taurus	COI_cow_F	CGACCAAATCTACAACGTAG	102	61
Bos taurus	COI_cow_R	GGAACAAGTCAGTTACCG	102	61
Cervus elaphus and Cervus nippon	COI_deer_F	CTGCTTGGAGATGACCAAATT	125	59
Cervus elaphus and Cervus nippon	COI_deer_R	CCAATTATTAGGGGAACTAGTCAA	125	59
Ovis aries	COI_sheep_F	GGCAACTGACTAGTTCCT	117	59
Ovis aries	COI_sheep_R	CATAGAGGATGCTAGGAGTAAC	117	59

ISSN: 1749-8546