Effective authentication of Placenta Hominis

Table 3 Summary of iodine test, detection of hCG and progesterone hormones and diagnostic PCR results

Sample code	Iodine test^a	HCG detection^b	Progesterone conc.^c (ng/ml)	Diagnostic PCR^d
Sample code	Iodine test^a	HCG detection^b	Progesterone conc.^c (ng/ml)	Human	Cow	Deer	Sheep
PH01	N	Y	544.19 ± 120.76	Y	N	N	N
PH02	N	Y	435.17 ± 86.80	Y	N	N	N
PH03	N	Y	265.34 ± 49.59	Y	N	N	N
PH04	N	Y	461.48 ± 78.98	Y	N	N	N
PH05	N	Y	316.37 ± 52.92	Y	N	N	N
PH06	Y	Y	843.43 ± 93.75	Y	N	N	N
PH07	Y	N	Not detected	N	N	N	N
PH08	Y	N	Not detected	N	N	N	N
PH09	Y	N	Not detected	N	N	N	N
PH10	Y	N	Not detected	N	N	N	N

^aIn iodine test, Y and N indicate positive result with observable color change from brown to blue, and negative result without color change (i.e. brown), respectively
^bIn hCG detection, Y represents the presence of two pink bands at the Test (T) and Control (C) sections of pregnancy test strip, while N represents the presence of a single pink band at the C section only
^cAssay buffer was set as blank and the data represent mean ± standard deviation (n = 3)
^dIn diagnostic PCR, Y and N represent the presence and absence of amplified products, respectively