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Table 3 Summary of iodine test, detection of hCG and progesterone hormones and diagnostic PCR results

From: Effective authentication of Placenta Hominis

Sample code

Iodine testa

HCG detectionb

Progesterone conc.c (ng/ml)

Diagnostic PCRd

Human

Cow

Deer

Sheep

PH01

N

Y

544.19 ± 120.76

Y

N

N

N

PH02

N

Y

435.17 ± 86.80

Y

N

N

N

PH03

N

Y

265.34 ± 49.59

Y

N

N

N

PH04

N

Y

461.48 ± 78.98

Y

N

N

N

PH05

N

Y

316.37 ± 52.92

Y

N

N

N

PH06

Y

Y

843.43 ± 93.75

Y

N

N

N

PH07

Y

N

Not detected

N

N

N

N

PH08

Y

N

Not detected

N

N

N

N

PH09

Y

N

Not detected

N

N

N

N

PH10

Y

N

Not detected

N

N

N

N

  1. aIn iodine test, Y and N indicate positive result with observable color change from brown to blue, and negative result without color change (i.e. brown), respectively
  2. bIn hCG detection, Y represents the presence of two pink bands at the Test (T) and Control (C) sections of pregnancy test strip, while N represents the presence of a single pink band at the C section only
  3. cAssay buffer was set as blank and the data represent mean ± standard deviation (n = 3)
  4. dIn diagnostic PCR, Y and N represent the presence and absence of amplified products, respectively