Fig. 4

RAW 264.7 cells were pretreated with Siegesbeckia pubescens Makino (SP) (n = 3) for 4 h before Pam₃CSK₄ (200 ng/mL) stimulation for another 12 h. NO was captured by Leica TCS SP8 laser scanning confocal microscope with 5 μM DAF-FM diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate). CU-CPT22 (CU) is selected as the positive control