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Fig. 1 | Chinese Medicine

Fig. 1

From: Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography–mass spectrometry

Fig. 1

SDS-PAGE analysis of β2-AR. Lane 1, collection from Quaternary Sepharose Fast Flow column gradiently eluted by 18–26% phosphate buffer; Lane 2, collection from Quaternary Sepharose Fast Flow column gradiently eluted by 26–40% phosphate buffer; Lane 3, protein molecular weight marker (the molecular weights from top to bottom were 116.0, 66.2, 45.0, 35.0, 25.0, 18.4 and 14.4 kDa, respectively); Lane 4, collection from Quaternary Sepharose Fast Flow column gradiently eluted by 40–50% phosphate buffer. The concentration of phosphate buffer was 20 mM with the presence of 0.8 M NaCl

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