Fig. 1
Cell viability of bEnd.3 cell under Aβ1-42 oligomers treatment (a). Permeability and TEER in bEnd.3 monoculture and co-culture with C8-D1A cells (b, c). Immunofluorescent staining of tight junction proteins (ZO-1, Claudin-5), adherens junction protein (PECAM-1), and cytoskeleton F-actin (d). Data represent mean ± SEM of three independent experiments each performed in triplicate (n = 3). *P < 0.05 vs bEnd.3 group; #P < 0.05 vs co-culture group. Cells were imaged by IN Cell Analyzer 2000 system (general electric) and analyzed by ImageJ software