Fig. 6
Scavenging intracellular ROS by pretreating NAC (5 mM) blocked the sensitizing effects of SKN in PTX-induced cytotoxicity and apoptosis in A2780/PTX cells after 24 h of treatment. a A2780/PTX cells were seeded at 5000/well in 96-well plates and treated with SKN (1 μM), PTX (1 μM) or the combination for 24 h. Cell viability was detected using SRB assay. b A2780/PTX cells were seeded at 2 × 105/well in 6-well plates and treated with SKN (1 μM), PTX (1 μM) or the combination for 24 h. Apoptosis was analyzed using Annexin V-FITC/PI co-staining and flow cytometry analysis. c Apoptosis rate was further calculated as early and late apoptotic cell population. d Western blot was conducted to detect the expression of PARP and its cleaved form