Fig. 4

Validation of differentially expressed genes by using quantitative RT-PCR. Expression levels of nine genes were measured in HepG2 cells treated with C. pilosula or A. membranaceus (3 mg/mL) for 24 h by using qRT-PCR. Internal control: ACTB. The relative expression levels of genes were normalized to the non-treated group. All experiments were repeated at least three times and results are presented as mean ± SEM. *P < 0.05, Student t test