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Table 1 Precursor/product ion pairs and parameters for MRM of compounds used in this study

From: Simultaneous UPLC–TQ-MS/MS determination of six active components in rat plasma: application in the pharmacokinetic study of Cyclocarya paliurus leaves

Analyte RT (min) Precursor ion species MRM transition Frag. (V) CE (eV) Dwell time (ms) Ionization mode
Precursor ion → Product ion (m/z)
Neochlorogenic acid 1.97 (M−H) 353.1 → 191.0 Quantifier 104 9 20 Negative
    353.1 → 135.0 Qualifier 104 29 20  
Chlorogenic acid 2.45 (M−H) 353.1 → 191.0 Quantifier 104 9 20 Negative
    353.1 → 135.0 Qualifier 104 29 20  
Quercetin-3-O-glucuronide 3.44 (M−H) 477.1 → 301.1 Quantifier 110 17 20 Negative
    477.1 → 151.0 Qualifier 110 37 20  
Kaempferol-3-O-rhamnoside 4.04 (M−H) 431.1 → 285.0 Quantifier 170 13 20 Negative
    431.1 → 255.0 Qualifier 170 37 20  
Quercetin 4.50 (M−H) 301.0 → 151.0 Quantifier 114 17 20 Negative
    301.0 → 179.0 Qualifier 114 9 20  
Kaempferol 5.03 (M−H) 285.0 → 185.1 Quantifier 170 27 20 Negative
    285.0 → 117.0 Qualifier 170 43 20  
Naringin (I.S.) 3.80 (M−H) 579.2 → 271.0 Quantifier 150 15 20 Negative
    579.2 → 151.0 Qualifier 150 30 20