Analyte | Nominal concentration (ng/ml) | Intraday | Interday | Matrix effect (%, mean ± SD) | Extraction recovery (%, mean ± SD) | ||
---|---|---|---|---|---|---|---|
Precision (RSD, %) | Accuracy (RE, %) | Precision (RSD, %) | Accuracy (RE, %) | ||||
Neochlorogenic acid | 120 | 12.9 | − 11.3 | 11.2 | − 10.8 | 110.5 ± 12.3 | 109.2 ± 13.5 |
1200 | 11.8 | 9.3 | 9.9 | 12.5 | 102.2 ± 5.6 | 84.6 ± 12.9 | |
12,000 | 9.3 | − 9.2 | 9.6 | 14.1 | 101.1 ± 12.1 | 80.5 ± 10.5 | |
Chlorogenic acid | 120 | 10.2 | 10.4 | 8.8 | 13.4 | 80.2 ± 12.7 | 106.8 ± 9.6 |
1200 | 10.1 | − 7.9 | 4.6 | − 11.0 | 91.2 ± 15.3 | 90.1 ± 9.8 | |
12,000 | 9.5 | 10.9 | 9.2 | 9.6 | 99.4 ± 11.0 | 80.1 ± 13.4 | |
Quercetin-3-O-glucuronide | 40 | 12.5 | 13.0 | 5.8 | − 12.6 | 115.1 ± 13.5 | 96.5 ± 12.6 |
400 | 12.6 | − 5.6 | 4.6 | 13.5 | 101.6 ± 12.9 | 116.3 ± 6.9 | |
4000 | 10.5 | 8.3 | 8.4 | 12.4 | 110.1 ± 12.4 | 115.4 ± 9.5 | |
Kaempferol-3-O-rhamnoside | 5 | 8.9 | 13.1 | 7.6 | 13.6 | 110.1 ± 6.3 | 93.0 ± 13.5 |
50 | 8.2 | 15.1 | 4.8 | 4.9 | 115.6 ± 9.2 | 86.5 ± 14.8 | |
500 | 10.5 | − 13.2 | 6.1 | − 8.2 | 119.2 ± 9.4 | 94.4 ± 11.0 | |
Quercetin | 1.5 | 12.6 | − 12.5 | 6.5 | − 12.8 | 120.4 ± 11.3 | 89.5 ± 14.8 |
15 | 10.9 | 13.6 | 8.3 | 10.4 | 119.5 ± 12.0 | 82.6 ± 13.9 | |
150 | 10.7 | 5.3 | 12.2 | 8.2 | 112.5 ± 10.1 | 89.0 ± 14.8 | |
Kaempferol | 20 | 6.8 | 12.5 | 5.4 | − 12.5 | 83.5 ± 5.4 | 81.9 ± 11.8 |
200 | 7.8 | − 12.3 | 5.7 | − 9.2 | 88.6 ± 9.9 | 86.6 ± 15.1 | |
2000 | 10.2 | − 9.2 | 7.6 | 10.4 | 94.7 ± 8.4 | 83.5 ± 11.4 |