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Table 3 Precision, accuracy, extraction recovery, matrix effect of the six components in rat plasma samples

From: Simultaneous UPLC–TQ-MS/MS determination of six active components in rat plasma: application in the pharmacokinetic study of Cyclocarya paliurus leaves

Analyte

Nominal concentration (ng/ml)

Intraday

Interday

Matrix effect (%, mean ± SD)

Extraction recovery (%, mean ± SD)

Precision (RSD, %)

Accuracy (RE, %)

Precision (RSD, %)

Accuracy (RE, %)

Neochlorogenic acid

120

12.9

− 11.3

11.2

− 10.8

110.5 ± 12.3

109.2 ± 13.5

1200

11.8

9.3

9.9

12.5

102.2 ± 5.6

84.6 ± 12.9

12,000

9.3

− 9.2

9.6

14.1

101.1 ± 12.1

80.5 ± 10.5

Chlorogenic acid

120

10.2

10.4

8.8

13.4

80.2 ± 12.7

106.8 ± 9.6

1200

10.1

− 7.9

4.6

− 11.0

91.2 ± 15.3

90.1 ± 9.8

12,000

9.5

10.9

9.2

9.6

99.4 ± 11.0

80.1 ± 13.4

Quercetin-3-O-glucuronide

40

12.5

13.0

5.8

− 12.6

115.1 ± 13.5

96.5 ± 12.6

400

12.6

− 5.6

4.6

13.5

101.6 ± 12.9

116.3 ± 6.9

4000

10.5

8.3

8.4

12.4

110.1 ± 12.4

115.4 ± 9.5

Kaempferol-3-O-rhamnoside

5

8.9

13.1

7.6

13.6

110.1 ± 6.3

93.0 ± 13.5

50

8.2

15.1

4.8

4.9

115.6 ± 9.2

86.5 ± 14.8

500

10.5

− 13.2

6.1

− 8.2

119.2 ± 9.4

94.4 ± 11.0

Quercetin

1.5

12.6

− 12.5

6.5

− 12.8

120.4 ± 11.3

89.5 ± 14.8

15

10.9

13.6

8.3

10.4

119.5 ± 12.0

82.6 ± 13.9

150

10.7

5.3

12.2

8.2

112.5 ± 10.1

89.0 ± 14.8

Kaempferol

20

6.8

12.5

5.4

− 12.5

83.5 ± 5.4

81.9 ± 11.8

200

7.8

− 12.3

5.7

− 9.2

88.6 ± 9.9

86.6 ± 15.1

2000

10.2

− 9.2

7.6

10.4

94.7 ± 8.4

83.5 ± 11.4