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Table 3 Precision, accuracy, extraction recovery, matrix effect of the six components in rat plasma samples

From: Simultaneous UPLC–TQ-MS/MS determination of six active components in rat plasma: application in the pharmacokinetic study of Cyclocarya paliurus leaves

Analyte Nominal concentration (ng/ml) Intraday Interday Matrix effect (%, mean ± SD) Extraction recovery (%, mean ± SD)
Precision (RSD, %) Accuracy (RE, %) Precision (RSD, %) Accuracy (RE, %)
Neochlorogenic acid 120 12.9 − 11.3 11.2 − 10.8 110.5 ± 12.3 109.2 ± 13.5
1200 11.8 9.3 9.9 12.5 102.2 ± 5.6 84.6 ± 12.9
12,000 9.3 − 9.2 9.6 14.1 101.1 ± 12.1 80.5 ± 10.5
Chlorogenic acid 120 10.2 10.4 8.8 13.4 80.2 ± 12.7 106.8 ± 9.6
1200 10.1 − 7.9 4.6 − 11.0 91.2 ± 15.3 90.1 ± 9.8
12,000 9.5 10.9 9.2 9.6 99.4 ± 11.0 80.1 ± 13.4
Quercetin-3-O-glucuronide 40 12.5 13.0 5.8 − 12.6 115.1 ± 13.5 96.5 ± 12.6
400 12.6 − 5.6 4.6 13.5 101.6 ± 12.9 116.3 ± 6.9
4000 10.5 8.3 8.4 12.4 110.1 ± 12.4 115.4 ± 9.5
Kaempferol-3-O-rhamnoside 5 8.9 13.1 7.6 13.6 110.1 ± 6.3 93.0 ± 13.5
50 8.2 15.1 4.8 4.9 115.6 ± 9.2 86.5 ± 14.8
500 10.5 − 13.2 6.1 − 8.2 119.2 ± 9.4 94.4 ± 11.0
Quercetin 1.5 12.6 − 12.5 6.5 − 12.8 120.4 ± 11.3 89.5 ± 14.8
15 10.9 13.6 8.3 10.4 119.5 ± 12.0 82.6 ± 13.9
150 10.7 5.3 12.2 8.2 112.5 ± 10.1 89.0 ± 14.8
Kaempferol 20 6.8 12.5 5.4 − 12.5 83.5 ± 5.4 81.9 ± 11.8
200 7.8 − 12.3 5.7 − 9.2 88.6 ± 9.9 86.6 ± 15.1
2000 10.2 − 9.2 7.6 10.4 94.7 ± 8.4 83.5 ± 11.4