Table 4 The stability of the six components in rat plasma samples
Analytes | Nominal concentration (ng/ml) | Frozen for 15 days at − 20 °C | Three-free-thaw cycles | Auto-sampler for 24 h | |||
|---|---|---|---|---|---|---|---|
Precision (RSD, %) | Accuracy (RE, %) | Precision (RSD, %) | Accuracy (RE, %) | Precision (RSD, %) | Accuracy (RE, %) | ||
Neochlorogenic acid | 120 | 12.24 | 12.06 | 14.24 | − 12.82 | 11.20 | − 11.03 |
1200 | 9.39 | − 10.45 | 10.39 | − 12.41 | 6.35 | 10.03 | |
12,000 | 9.36 | 7.35 | 10.36 | 5.35 | 9.33 | 13.02 | |
Chlorogenic acid | 120 | 12.03 | − 9.60 | 14.03 | − 15.01 | 13.36 | 12.53 |
1200 | 10.02 | 8.03 | 11.63 | 14.06 | 12.59 | − 9.36 | |
12,000 | 6.50 | − 8.59 | 14.30 | 14.03 | 9.36 | 8.03 | |
Quercetin-3-O-glucuronide | 40 | 13.02 | − 11.20 | 13.63 | 5.16 | 7.26 | − 12.25 |
400 | 8.20 | 14.06 | 10.96 | − 11.52 | 5.88 | 4.62 | |
4000 | 9.56 | 10.12 | 15.05 | 8.29 | 9.26 | − 9.13 | |
Kaempferol-3-O-rhamnoside | 5 | 14.01 | 12.58 | 9.86 | − 9.86 | 3.89 | − 9.59 |
50 | 14.23 | 12.63 | 8.16 | − 11.05 | 12.50 | 9.25 | |
500 | 15.20 | 15.03 | 12.03 | 7.16 | 6.23 | − 6.33 | |
Quercetin | 1.5 | 10.36 | − 12.3 | 7.59 | 7.68 | 9.51 | 14.59 |
15 | 12.60 | − 10.52 | 8.16 | 11.20 | 5.62 | 6.09 | |
150 | 10.24 | 9.35 | 12.14 | − 15.01 | 8.45 | 11.13 | |
Kaempferol | 20 | 14.05 | 9.18 | 9.68 | 14.10 | 9.66 | − 15.01 |
200 | 10.02 | − 11.05 | 15.11 | − 15.02 | 7.33 | 11.06 | |
2000 | 9.05 | 11.56 | 9.13 | 11.30 | 6.03 | 11.42 | |