Table 1 Coded and uncoded values of parameters
Process | Parameters | Symbols | Coded values | ||
|---|---|---|---|---|---|
− 1 | 0 | 1 | |||
Ethanol extraction | Ethanol solution concentration for extraction (v/v %) | X1 | 80 | 85 | 90 |
Extraction time (h) | X2 | 6.0 | 8.0 | 10.0 | |
Ethanol solution volume (mL) | X3 | 600 | 800 | 1000 | |
Concentration | Volume of concentrated extract (mL) | X4 | 100 | 150 | 200 |
Water precipitation | Water added for water precipitation (mL) | X5 | 300 | 400 | 500 |
Column chromatography | Sampling flow rate (BV/h) | X6 | 1.0 | 1.5 | 2.0 |
Washing flow rate (BV/h) | X7 | 1.0 | 1.5 | 2.0 | |
Water volume (BV) | X8 | 1.0 | 2.0 | 3.0 | |
Ethanol solution concentration for elution (v/v %) | X9 | 55 | 60 | 65 | |
Elution time (min) | X10 | 120 | 150 | 180 | |