Fig. 3
From: Specific DNA identification of Pheretima in the Naoxintong capsule
The sensitivity test of NXTC using primers MF2R2. DNA templates of NXTC (50 ng/μl) (50) were fivefold serial-diluted (51 to 57) and were amplified using specific primers MF2R2. The suggested detection limit of DNA concentration of NXTC was 2 ng/μl, which was indicated by red arrow. Pheretima-deficient NXTC (T40) was made as the negative control. M:DNA ladders, N:PCR without DNA template