Table 2 Primers for PCR amplification and sequencing
From: Specific DNA identification of Pheretima in the Naoxintong capsule
Primer name | Sequence (5′ → 3′) | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|
Universal primers of COI [22] | |||
LCO1490 | GGTCAACAAATCATAAAGATATTGG | 650 | 40 |
HCO2198 | TAAACTTTCAGGGTGACCAAAAAATCA | ||
MF2R2 | |||
COI Metaphire F2 | TTAGTGTCGTCCGCCGCAGTT | 232 | 58 |
COI Metaphire R2 | CTACTGCCCACACAAATAGTGGG | ||
AF3R1 | |||
COI AA F3 | TTTGGAAACTGACTGCTCCCA | 247 | 55 |
COI AA R1 | CTAAAATTGATGAGGCACCC | ||